##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	HBR_Rep1_Aligned.sortedByCoord.out.bam
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1218
Sequences flagged as poor quality	0
Sequence length	36-100
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83825944170772	34.0	31.0	34.0	31.0	34.0
2	32.90968801313629	34.0	31.0	34.0	31.0	34.0
3	32.97865353037767	34.0	31.0	34.0	31.0	34.0
4	36.24794745484401	37.0	37.0	37.0	35.0	37.0
5	36.301313628899834	37.0	37.0	37.0	35.0	37.0
6	36.270114942528735	37.0	37.0	37.0	35.0	37.0
7	36.23973727422003	37.0	37.0	37.0	35.0	37.0
8	36.235632183908045	37.0	37.0	37.0	35.0	37.0
9	38.20361247947455	39.0	39.0	39.0	37.0	39.0
10-11	38.05172413793103	39.0	39.0	39.0	37.0	39.0
12-13	38.00369458128079	39.0	38.5	39.0	35.0	39.0
14-15	39.57307060755336	41.0	40.0	41.0	36.5	41.0
16-17	39.64039408866995	41.0	40.0	41.0	37.0	41.0
18-19	39.51559934318555	41.0	39.5	41.0	36.5	41.0
20-21	39.504515599343186	41.0	39.5	41.0	36.0	41.0
22-23	39.35714285714286	41.0	39.0	41.0	36.0	41.0
24-25	39.35426929392446	41.0	39.0	41.0	36.0	41.0
26-27	39.29228243021346	41.0	39.0	41.0	36.0	41.0
28-29	39.183908045977006	40.5	39.0	41.0	35.5	41.0
30-31	39.05008210180624	40.0	39.0	41.0	35.5	41.0
32-33	38.96510673234811	40.0	38.0	41.0	35.0	41.0
34-35	38.820197044334975	40.0	38.0	41.0	35.0	41.0
36-37	38.71648882443177	40.0	38.0	41.0	35.0	41.0
38-39	38.59580592105263	40.0	38.0	41.0	34.5	41.0
40-41	38.46340460526316	40.0	38.0	41.0	33.5	41.0
42-43	38.32360197368421	40.0	38.0	41.0	33.5	41.0
44-45	38.35197368421052	40.0	38.0	41.0	33.5	41.0
46-47	38.31661184210526	40.0	38.0	41.0	33.5	41.0
48-49	38.40213815789474	40.0	37.0	41.0	33.5	41.0
50-51	37.540296052631575	39.0	36.0	40.5	32.0	41.0
52-53	37.48600823045267	39.0	35.5	40.5	32.5	41.0
54-55	37.788065843621396	39.0	36.0	41.0	33.0	41.0
56-57	37.68051064060583	39.0	35.5	41.0	33.0	41.0
58-59	37.31863149216818	39.0	35.0	41.0	33.0	41.0
60-61	37.115416323165704	38.5	35.0	40.5	32.5	41.0
62-63	36.66735366859027	37.0	35.0	40.0	32.5	41.0
64-65	36.23289365210223	37.0	35.0	39.0	32.0	41.0
66-67	35.78524319868096	36.0	35.0	39.0	31.0	41.0
68-69	35.42839530650009	36.0	35.0	38.5	31.0	40.5
70-71	35.180165289256195	35.0	35.0	37.0	31.5	39.5
72-73	34.76570247933884	35.0	34.0	37.0	31.0	39.0
74-75	34.470723107454575	35.0	34.0	36.0	30.0	39.0
76-77	33.73105332896267	35.0	33.0	35.0	29.5	37.0
78-79	34.098679572578405	35.0	34.0	35.0	30.5	37.0
80-81	33.9681750799828	35.0	34.0	35.0	30.5	36.5
82-83	33.71432389695079	35.0	34.0	35.0	30.0	36.0
84-85	33.47258187730844	35.0	33.0	35.0	30.0	36.0
86-87	33.58844979933223	35.0	33.5	35.0	30.0	35.5
88-89	33.403788304272716	35.0	33.5	35.0	30.0	35.0
90-91	33.438448566610454	35.0	34.0	35.0	30.0	35.0
92-93	33.3409282700422	35.0	33.0	35.0	29.5	35.0
94-95	33.37474619325581	35.0	34.0	35.0	29.5	35.0
96-97	33.54448870649718	35.0	33.5	35.0	30.5	35.0
98-99	33.50518506347008	35.0	33.5	35.0	30.5	35.0
100	33.51487710219922	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0483091787439562
1101	2	1.3710144927536163
1101	3	1.165942028985505
1101	4	1.0434782608695627
1101	5	0.8096618357487912
1101	6	0.8444444444444414
1101	7	0.9787439613526487
1101	8	0.7258454106280183
1101	9	0.8594202898550733
1101	10-11	0.9259661835748858
1101	12-13	0.8524154589371946
1101	14-15	1.507246376811601
1101	16-17	1.0252415458937207
1101	18-19	0.9002415458937207
1101	20-21	1.69722222222223
1101	22-23	-3.4574879227053117
1101	24-25	0.9722222222222214
1101	26-27	1.5660628019323681
1101	28-29	1.7230676328502383
1101	30-31	2.148792270531402
1101	32-33	2.500603864734309
1101	34-35	1.747584541062814
1101	36-37	2.4879227053139985
1101	38-39	0.8695652173913047
1101	40-41	0.9019323671497688
1101	42-43	1.9066425120772976
1101	44-45	1.6274154589371932
1101	46-47	-1.7865942028985557
1101	48-49	-1.9960144927536234
1101	50-51	-0.5658212560386531
1101	52-53	-2.883333333333333
1101	54-55	-0.6544685990338124
1101	56-57	-0.8411835748792242
1101	58-59	0.4402173913043441
1101	60-61	0.8021739130434824
1101	62-63	0.6995169082125656
1101	64-65	-1.731159420289849
1101	66-67	-4.0288647342995105
1101	68-69	-0.8679951690821284
1101	70-71	3.1124396135265684
1101	72-73	-3.775241545893728
1101	74-75	-5.416183574879227
1101	76-77	0.5793478260869591
1101	78-79	3.02041062801932
1101	80-81	2.798671497584543
1101	82-83	3.07826086956522
1101	84-85	1.8690821256038674
1101	86-87	-0.14528985507246261
1101	88-89	2.410748792270539
1101	90-91	1.6557971014492807
1101	92-93	2.4100241545893795
1101	94-95	1.8175120772946798
1101	96-97	2.4692028985507335
1101	98-99	2.573067632850247
1101	100	9.323671497584542
1102	1	0.7149758454106205
1102	2	1.3710144927536163
1102	3	0.16594202898550492
1102	4	1.0434782608695627
1102	5	0.8096618357487912
1102	6	0.8444444444444414
1102	7	0.9787439613526487
1102	8	0.7258454106280183
1102	9	0.8594202898550733
1102	10-11	0.7592995169082144
1102	12-13	0.9357487922705232
1102	14-15	1.007246376811601
1102	16-17	1.2752415458937207
1102	18-19	0.48357487922704934
1102	20-21	1.3638888888888943
1102	22-23	1.2091787439613597
1102	24-25	0.55555555555555
1102	26-27	1.7327294685990324
1102	28-29	1.4730676328502383
1102	30-31	1.9821256038647306
1102	32-33	2.3339371980676376
1102	34-35	2.0809178743961425
1102	36-37	2.2379227053139985
1102	38-39	1.2028985507246333
1102	40-41	0.15193236714976877
1102	42-43	1.573309178743969
1102	44-45	1.7940821256038646
1102	46-47	1.4634057971014443
1102	48-49	2.420652173913041
1102	50-51	2.2675120772946755
1102	52-53	3.7833333333333314
1102	54-55	2.5955314009661876
1102	56-57	2.9921497584541115
1102	58-59	-3.72644927536232
1102	60-61	2.135507246376818
1102	62-63	-0.38381642512076297
1102	64-65	1.768840579710151
1102	66-67	1.054468599033818
1102	68-69	2.3820048309178716
1102	70-71	1.695772946859904
1102	72-73	2.3914251207729436
1102	74-75	2.6671497584541015
1102	76-77	2.9126811594202877
1102	78-79	0.6037439613526558
1102	80-81	0.13200483091787163
1102	82-83	-0.42173913043477995
1102	84-85	0.28574879227053174
1102	86-87	-2.4786231884057948
1102	88-89	-0.25591787439613256
1102	90-91	-0.17753623188404788
1102	92-93	-1.3399758454106205
1102	94-95	-0.5158212560386559
1102	96-97	0.8025362318840621
1102	98-99	-1.7602657004830888
1102	100	4.823671497584542
1104	1	1.0483091787439562
1104	2	0.3710144927536163
1104	3	0.16594202898550492
1104	4	1.0434782608695627
1104	5	0.8096618357487912
1104	6	0.8444444444444414
1104	7	0.9787439613526487
1104	8	-1.2741545893719817
1104	9	0.8594202898550733
1104	10-11	0.9259661835748858
1104	12-13	0.10241545893719461
1104	14-15	0.5072463768116009
1104	16-17	1.2752415458937207
1104	18-19	1.6502415458937207
1104	20-21	1.69722222222223
1104	22-23	1.5425120772946883
1104	24-25	1.7222222222222214
1104	26-27	2.066062801932368
1104	28-29	0.9730676328502383
1104	30-31	2.148792270531402
1104	32-33	2.000603864734309
1104	34-35	2.247584541062814
1104	36-37	2.7379227053139985
1104	38-39	1.8695652173913047
1104	40-41	1.6519323671497688
1104	42-43	2.4066425120772976
1104	44-45	3.127415458937193
1104	46-47	3.4634057971014443
1104	48-49	3.2539855072463766
1104	50-51	1.934178743961347
1104	52-53	2.116666666666667
1104	54-55	2.5955314009661876
1104	56-57	0.6588164251207758
1104	58-59	3.440217391304344
1104	60-61	3.8021739130434824
1104	62-63	2.9495169082125656
1104	64-65	3.268840579710151
1104	66-67	5.2211352657004895
1104	68-69	5.382004830917872
1104	70-71	6.362439613526568
1104	72-73	4.724758454106272
1104	74-75	3.833816425120773
1104	76-77	-1.4206521739130409
1104	78-79	4.77041062801932
1104	80-81	3.798671497584543
1104	82-83	4.07826086956522
1104	84-85	3.6190821256038674
1104	86-87	2.8547101449275374
1104	88-89	2.910748792270539
1104	90-91	2.1557971014492807
1104	92-93	1.6600241545893795
1104	94-95	2.31751207729468
1104	96-97	2.4692028985507335
1104	98-99	-0.42693236714975313
1104	100	-25.67632850241546
1105	1	1.0483091787439562
1105	2	1.3710144927536163
1105	3	1.165942028985505
1105	4	1.0434782608695627
1105	5	0.8096618357487912
1105	6	0.8444444444444414
1105	7	0.9787439613526487
1105	8	0.7258454106280183
1105	9	0.8594202898550733
1105	10-11	0.4259661835748858
1105	12-13	1.1024154589371946
1105	14-15	1.007246376811601
1105	16-17	0.7752415458937207
1105	18-19	-2.3497584541062793
1105	20-21	-5.30277777777777
1105	22-23	-0.4574879227053117
1105	24-25	-0.27777777777777857
1105	26-27	1.5660628019323681
1105	28-29	1.9730676328502383
1105	30-31	-0.851207729468598
1105	32-33	1.000603864734309
1105	34-35	1.247584541062814
1105	36-37	-1.2620772946860015
1105	38-39	-1.1304347826086953
1105	40-41	-2.3480676328502312
1105	42-43	-10.093357487922702
1105	44-45	-1.8725845410628068
1105	46-47	0.9634057971014443
1105	48-49	1.2539855072463766
1105	50-51	-2.065821256038653
1105	52-53	-0.38333333333333286
1105	54-55	0.09553140096618762
1105	56-57	0.15881642512077576
1105	58-59	-2.059782608695656
1105	60-61	0.3021739130434824
1105	62-63	1.9495169082125656
1105	64-65	3.268840579710151
1105	66-67	2.7211352657004895
1105	68-69	-8.117995169082128
1105	70-71	-5.137560386473432
1105	72-73	1.2247584541062722
1105	74-75	1.333816425120773
1105	76-77	2.579347826086959
1105	78-79	-2.72958937198068
1105	80-81	1.298671497584543
1105	82-83	0.57826086956522
1105	84-85	2.1190821256038674
1105	86-87	1.3547101449275374
1105	88-89	-2.089251207729461
1105	90-91	2.1557971014492807
1105	92-93	1.1600241545893795
1105	94-95	2.31751207729468
1105	96-97	0.4692028985507335
1105	98-99	2.573067632850247
1105	100	9.323671497584542
1106	1	-0.45169082125604376
1106	2	-0.1289855072463837
1106	3	1.165942028985505
1106	4	0.043478260869562746
1106	5	0.8096618357487912
1106	6	0.8444444444444414
1106	7	0.9787439613526487
1106	8	-0.27415458937198167
1106	9	0.8594202898550733
1106	10-11	0.9259661835748858
1106	12-13	1.1024154589371946
1106	14-15	1.007246376811601
1106	16-17	1.0252415458937207
1106	18-19	1.4002415458937207
1106	20-21	1.44722222222223
1106	22-23	1.7925120772946883
1106	24-25	1.9722222222222214
1106	26-27	2.316062801932368
1106	28-29	1.9730676328502383
1106	30-31	0.398792270531402
1106	32-33	2.000603864734309
1106	34-35	0.7475845410628139
1106	36-37	1.4879227053139985
1106	38-39	1.8695652173913047
1106	40-41	1.4019323671497688
1106	42-43	1.9066425120772976
1106	44-45	2.377415458937193
1106	46-47	3.2134057971014443
1106	48-49	3.2539855072463766
1106	50-51	2.684178743961347
1106	52-53	2.866666666666667
1106	54-55	2.3455314009661876
1106	56-57	2.1588164251207758
1106	58-59	2.440217391304344
1106	60-61	4.052173913043482
1106	62-63	2.4495169082125656
1106	64-65	2.518840579710151
1106	66-67	2.2211352657004895
1106	68-69	2.3820048309178716
1106	70-71	0.8624396135265684
1106	72-73	2.224758454106272
1106	74-75	1.833816425120773
1106	76-77	2.829347826086959
1106	78-79	1.7704106280193201
1106	80-81	1.298671497584543
1106	82-83	1.57826086956522
1106	84-85	0.11908212560386744
1106	86-87	1.3547101449275374
1106	88-89	2.160748792270539
1106	90-91	2.1557971014492807
1106	92-93	-2.5899758454106205
1106	94-95	-2.18248792270532
1106	96-97	1.2192028985507335
1106	98-99	2.573067632850247
1106	100	9.323671497584542
1107	1	1.0483091787439562
1107	2	0.8710144927536163
1107	3	1.165942028985505
1107	4	1.0434782608695627
1107	5	0.8096618357487912
1107	6	0.8444444444444414
1107	7	0.9787439613526487
1107	8	0.7258454106280183
1107	9	0.8594202898550733
1107	10-11	0.6759661835748858
1107	12-13	1.1024154589371946
1107	14-15	1.007246376811601
1107	16-17	1.2752415458937207
1107	18-19	1.6502415458937207
1107	20-21	1.69722222222223
1107	22-23	2.0425120772946883
1107	24-25	1.9722222222222214
1107	26-27	1.8160628019323681
1107	28-29	1.2230676328502383
1107	30-31	1.398792270531402
1107	32-33	2.000603864734309
1107	34-35	2.247584541062814
1107	36-37	2.7379227053139985
1107	38-39	2.3695652173913047
1107	40-41	2.6519323671497688
1107	42-43	2.6566425120772976
1107	44-45	2.877415458937193
1107	46-47	2.4634057971014443
1107	48-49	2.7539855072463766
1107	50-51	3.184178743961347
1107	52-53	3.616666666666667
1107	54-55	3.3455314009661876
1107	56-57	3.6588164251207758
1107	58-59	3.690217391304344
1107	60-61	4.302173913043482
1107	62-63	4.449516908212566
1107	64-65	5.018840579710151
1107	66-67	4.2211352657004895
1107	68-69	4.882004830917872
1107	70-71	3.3624396135265684
1107	72-73	2.724758454106272
1107	74-75	2.833816425120773
1107	76-77	2.579347826086959
1107	78-79	2.27041062801932
1107	80-81	2.298671497584543
1107	82-83	2.57826086956522
1107	84-85	1.8690821256038674
1107	86-87	1.8547101449275374
1107	88-89	2.410748792270539
1107	90-91	2.4057971014492807
1107	92-93	2.1600241545893795
1107	94-95	2.06751207729468
1107	96-97	2.4692028985507335
1107	98-99	2.573067632850247
1107	100	9.323671497584542
1108	1	-1.9516908212560438
1108	2	-1.6289855072463837
1108	3	1.165942028985505
1108	4	-5.956521739130437
1108	5	-1.1903381642512088
1108	6	-0.15555555555555856
1108	7	-1.0212560386473513
1108	8	0.7258454106280183
1108	9	0.8594202898550733
1108	10-11	0.9259661835748858
1108	12-13	1.1024154589371946
1108	14-15	1.507246376811601
1108	16-17	-2.2247584541062793
1108	18-19	0.15024154589372074
1108	20-21	-0.30277777777777004
1108	22-23	1.5425120772946883
1108	24-25	1.2222222222222214
1108	26-27	1.0660628019323681
1108	28-29	1.4730676328502383
1108	30-31	0.14879227053140198
1108	32-33	0.500603864734309
1108	34-35	2.247584541062814
1108	36-37	2.2379227053139985
1108	38-39	-0.13043478260869534
1108	40-41	2.1519323671497688
1108	42-43	0.9066425120772976
1108	44-45	2.627415458937193
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2211	74-75	4.333816425120773
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2313	70-71	-5.137560386473432
2313	72-73	0.47475845410627215
2313	74-75	0.3338164251207729
2313	76-77	-1.4206521739130409
2313	78-79	-3.72958937198068
2313	80-81	-0.701328502415457
2313	82-83	-2.67173913043478
2313	84-85	-3.6309178743961326
2313	86-87	-2.3952898550724626
2313	88-89	-2.339251207729461
2313	90-91	-0.09420289855071928
2313	92-93	-4.3399758454106205
2313	94-95	-8.93248792270532
2313	96-97	-5.0307971014492665
2313	98-99	-1.6769323671497531
2313	100	4.323671497584542
2314	1	1.0483091787439562
2314	2	1.3710144927536163
2314	3	1.165942028985505
2314	4	1.0434782608695627
2314	5	0.8096618357487912
2314	6	0.8444444444444414
2314	7	0.9787439613526487
2314	8	0.7258454106280183
2314	9	0.8594202898550733
2314	10-11	0.9259661835748858
2314	12-13	1.1024154589371946
2314	14-15	1.507246376811601
2314	16-17	1.2752415458937207
2314	18-19	1.6502415458937207
2314	20-21	1.69722222222223
2314	22-23	0.04251207729468831
2314	24-25	0.22222222222222143
2314	26-27	0.06606280193236813
2314	28-29	0.47306763285023834
2314	30-31	-0.851207729468598
2314	32-33	-0.49939613526569104
2314	34-35	0.7475845410628139
2314	36-37	-0.2620772946860015
2314	38-39	0.36956521739130466
2314	40-41	2.6519323671497688
2314	42-43	2.9066425120772976
2314	44-45	3.127415458937193
2314	46-47	2.9634057971014443
2314	48-49	2.2539855072463766
2314	50-51	-4.065821256038653
2314	52-53	-3.383333333333333
2314	54-55	1.5955314009661876
2314	56-57	0.6588164251207758
2314	58-59	-0.05978260869565588
2314	60-61	-0.1978260869565176
2314	62-63	-1.5504830917874344
2314	64-65	0.26884057971015096
2314	66-67	0.22113526570048947
2314	68-69	0.38200483091787163
2314	70-71	-0.1375603864734316
2314	72-73	1.7247584541062722
2314	74-75	1.833816425120773
2314	76-77	3.579347826086959
2314	78-79	2.27041062801932
2314	80-81	0.29867149758454303
2314	82-83	1.57826086956522
2314	84-85	1.6190821256038674
2314	86-87	1.8547101449275374
2314	88-89	2.410748792270539
2314	90-91	2.1557971014492807
2314	92-93	1.1600241545893795
2314	94-95	2.31751207729468
2314	96-97	2.4692028985507335
2314	98-99	2.573067632850247
2314	100	-25.67632850241546
2315	1	1.0483091787439562
2315	2	1.3710144927536163
2315	3	1.165942028985505
2315	4	1.0434782608695627
2315	5	0.8096618357487912
2315	6	0.8444444444444414
2315	7	0.9787439613526487
2315	8	0.7258454106280183
2315	9	-3.1405797101449267
2315	10-11	0.4259661835748858
2315	12-13	1.1024154589371946
2315	14-15	1.007246376811601
2315	16-17	1.2752415458937207
2315	18-19	1.6502415458937207
2315	20-21	1.69722222222223
2315	22-23	0.5425120772946883
2315	24-25	1.2222222222222214
2315	26-27	1.5660628019323681
2315	28-29	1.9730676328502383
2315	30-31	1.648792270531402
2315	32-33	0.500603864734309
2315	34-35	0.7475845410628139
2315	36-37	2.2379227053139985
2315	38-39	2.3695652173913047
2315	40-41	1.6519323671497688
2315	42-43	-0.5933574879227024
2315	44-45	-2.872584541062807
2315	46-47	-2.5365942028985557
2315	48-49	-2.7460144927536234
2315	50-51	-3.065821256038653
2315	52-53	-2.383333333333333
2315	54-55	-2.4044685990338124
2315	56-57	-2.8411835748792242
2315	58-59	-0.05978260869565588
2315	60-61	-1.6978260869565176
2315	62-63	-1.5504830917874344
2315	64-65	-0.731159420289849
2315	66-67	-0.27886473429951053
2315	68-69	0.38200483091787163
2315	70-71	0.3624396135265684
2315	72-73	1.2247584541062722
2315	74-75	1.333816425120773
2315	76-77	2.579347826086959
2315	78-79	1.7704106280193201
2315	80-81	1.798671497584543
2315	82-83	2.07826086956522
2315	84-85	2.1190821256038674
2315	86-87	1.8547101449275374
2315	88-89	2.410748792270539
2315	90-91	2.1557971014492807
2315	92-93	2.1600241545893795
2315	94-95	2.31751207729468
2315	96-97	2.4692028985507335
2315	98-99	3.073067632850247
2315	100	10.323671497584542
2316	1	0.4483091787439548
2316	2	0.3710144927536163
2316	3	0.5659420289855035
2316	4	0.6434782608695642
2316	5	0.40966183574879267
2316	6	-0.3555555555555614
2316	7	-0.02125603864735126
2316	8	0.1258454106280169
2316	9	0.45942028985507477
2316	10-11	0.725966183574883
2316	12-13	0.9024154589371918
2316	14-15	0.807246376811598
2316	16-17	1.1752415458937193
2316	18-19	0.7502415458937222
2316	20-21	1.19722222222223
2316	22-23	0.6425120772946897
2316	24-25	1.6222222222222271
2316	26-27	2.266062801932371
2316	28-29	1.9730676328502383
2316	30-31	1.7487922705313963
2316	32-33	0.7006038647343118
2316	34-35	1.747584541062814
2316	36-37	2.0379227053139957
2316	38-39	1.8695652173913047
2316	40-41	1.7519323671497702
2316	42-43	1.8066425120772962
2316	44-45	2.627415458937193
2316	46-47	2.863405797101443
2316	48-49	2.853985507246378
2316	50-51	1.434178743961347
2316	52-53	1.81666666666667
2316	54-55	1.2955314009661905
2316	56-57	2.6588164251207758
2316	58-59	2.940217391304344
2316	60-61	2.702173913043488
2316	62-63	2.849516908212564
2316	64-65	3.968840579710154
2316	66-67	3.4211352657004923
2316	68-69	4.482004830917866
2316	70-71	4.562439613526571
2316	72-73	4.924758454106275
2316	74-75	4.13381642512077
2316	76-77	4.479347826086965
2316	78-79	3.5704106280193173
2316	80-81	2.8986714975845445
2316	82-83	3.1782608695652215
2316	84-85	2.8190821256038703
2316	86-87	2.254710144927543
2316	88-89	2.1107487922705417
2316	90-91	2.1557971014492807
2316	92-93	2.1600241545893795
2316	94-95	2.117512077294677
2316	96-97	2.4692028985507335
2316	98-99	2.573067632850247
2316	100	9.657004830917877
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	5.0
25	1.0
26	4.0
27	9.0
28	11.0
29	16.0
30	14.0
31	33.0
32	21.0
33	33.0
34	57.0
35	82.0
36	192.0
37	368.0
38	335.0
39	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.086419753086425	10.205761316872428	10.041152263374485	46.666666666666664
2	27.339901477832512	15.517241379310345	33.579638752052546	23.563218390804597
3	27.011494252873565	21.592775041050906	21.34646962233169	30.049261083743843
4	28.101889893179948	27.115858668857847	19.556285949055052	25.22596548890715
5	30.870279146141215	28.48932676518883	20.689655172413794	19.950738916256157
6	24.958949096880133	27.914614121510674	22.167487684729064	24.958949096880133
7	21.26436781609195	17.733990147783253	35.05747126436782	25.94417077175698
8	20.525451559934318	20.607553366174056	25.36945812807882	33.497536945812804
9	22.33169129720854	19.293924466338257	28.899835796387517	29.47454844006568
10-11	27.257799671592775	24.589490968801314	20.77175697865353	27.380952380952383
12-13	25.59572719802794	21.15858668857847	25.184880854560394	28.0608052588332
14-15	26.683087027914613	22.742200328407225	24.589490968801314	25.985221674876847
16-17	27.66830870279146	22.824302134646963	23.316912972085387	26.190476190476193
18-19	27.914614121510674	23.357963875205254	22.701149425287355	26.026272577996718
20-21	26.51888341543514	24.09688013136289	23.809523809523807	25.57471264367816
22-23	26.51888341543514	22.701149425287355	23.727422003284072	27.05254515599343
24-25	25.69786535303777	23.891625615763548	21.510673234811165	28.899835796387517
26-27	26.108374384236456	23.6863711001642	22.126436781609197	28.078817733990146
28-29	26.231527093596057	21.34646962233169	24.09688013136289	28.32512315270936
30-31	25.82101806239737	23.973727422003286	22.988505747126435	27.216748768472904
32-33	25.215605749486652	23.901437371663246	21.642710472279262	29.24024640657084
34-35	28.119868637110017	21.469622331691298	23.39901477832512	27.011494252873565
36-37	27.567789646672146	22.637633525061627	22.3911257189811	27.403451109285125
38-39	25.986842105263158	24.136513157894736	22.574013157894736	27.302631578947366
40-41	26.356907894736842	25.041118421052634	22.16282894736842	26.439144736842106
42-43	27.220394736842106	22.574013157894736	23.4375	26.768092105263158
44-45	27.960526315789476	22.820723684210524	23.4375	25.78125
46-47	26.480263157894733	22.697368421052634	23.108552631578945	27.713815789473685
48-49	28.57730263157895	21.011513157894736	23.396381578947366	27.01480263157895
50-51	26.973684210526315	22.94407894736842	23.889802631578945	26.192434210526315
52-53	26.049382716049386	22.51028806584362	23.621399176954732	27.818930041152264
54-55	26.172839506172842	22.921810699588477	24.567901234567902	26.337448559670783
56-57	27.254013997529846	22.807739810621655	22.60189378344998	27.33635240839852
58-59	26.793075020610058	23.248145094806265	25.432811211871392	24.525968672712285
60-61	28.153338829348723	24.1962077493817	21.47568013190437	26.17477328936521
62-63	26.71063478977741	24.85572959604287	22.794723825226708	25.63891178895301
64-65	26.669414674361086	23.04204451772465	23.94888705688376	26.3396537510305
66-67	26.83429513602638	23.784006595218464	22.38252267106348	26.99917559769167
68-69	26.611570247933887	24.25619834710744	22.31404958677686	26.81818181818182
70-71	26.404958677685954	21.90082644628099	23.388429752066113	28.305785123966942
72-73	27.809917355371898	23.471074380165287	22.31404958677686	26.404958677685954
74-75	26.420572376607215	23.143923683119038	21.60929075072584	28.826213189547907
76-77	24.906444906444907	23.11850311850312	23.575883575883577	28.3991683991684
78-79	26.697209496043318	23.698458975426906	23.240316534777175	26.364014993752605
80-81	26.31578947368421	24.143692564745194	22.974101921470343	26.56641604010025
82-83	27.770807193642828	21.74822250104559	24.508573818485992	25.972396486825595
84-85	26.58015906236919	24.445374633737966	23.231477605692756	25.74298869820008
86-87	26.73931265716681	23.344509639564123	23.009220452640402	26.906957250628665
88-89	27.11578947368421	22.400000000000002	22.77894736842105	27.705263157894738
90-91	26.68634064080944	23.608768971332207	23.69308600337268	26.011804384485664
92-93	27.21518987341772	21.856540084388186	24.556962025316455	26.371308016877638
94-95	24.841571609632446	23.489649345162654	24.461343472750315	27.207435572454585
96-97	26.58984208279983	22.4498506188647	24.583866837387962	26.376440460947503
98-99	29.88826815642458	19.78584729981378	23.88268156424581	26.443202979515828
100	27.166882276843467	0.0	29.75420439844761	43.07891332470893
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	1.0
30	2.0
31	2.0
32	3.0
33	2.5
34	1.0
35	4.5
36	8.0
37	14.0
38	20.0
39	18.0
40	16.0
41	20.5
42	23.0
43	15.0
44	11.5
45	18.5
46	29.5
47	35.5
48	37.0
49	36.0
50	33.5
51	46.0
52	55.0
53	65.0
54	68.0
55	62.5
56	64.0
57	60.5
58	63.5
59	50.0
60	47.0
61	59.5
62	50.0
63	47.0
64	40.5
65	23.0
66	16.0
67	16.5
68	12.0
69	7.5
70	6.0
71	6.0
72	5.5
73	3.5
74	3.5
75	2.0
76	2.0
77	2.5
78	1.0
79	0.0
80	0.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24630541871921183
2	0.0
3	0.0
4	0.08210180623973727
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.08210180623973727
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.041050903119868636
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.12381345439537762
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
36-37	2.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	1.0
52-53	0.0
54-55	0.0
56-57	2.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.0
68-69	2.0
70-71	0.0
72-73	3.0
74-75	4.0
76-77	2.0
78-79	3.0
80-81	2.0
82-83	1.0
84-85	1.0
86-87	6.0
88-89	2.0
90-91	1.0
92-93	1.0
94-95	7.0
96-97	46.0
98-99	358.0
100-101	773.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.4696223316913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.51063829787235	93.10344827586206
2	3.3191489361702122	6.403940886699508
3	0.1702127659574468	0.49261083743842365
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGGAACTCCGTCTCGTTGCAGCAGTATTTGAAGACCGTGTTGTTATGGT	3	0.24630541871921183	No Hit
CTTCGATCAGAAGGTCCACAATGTGTCCTTCGCCTTTGAGCTGATGCTGG	3	0.24630541871921183	No Hit
GTAGGTTTCTGTGGTTTAAGCCATTCAGTCTGTGGTCACATGTTACAGTA	2	0.16420361247947454	No Hit
CTCCCTTCTTGCCTCTCCAGACGTGTTCCCCGAGGAGATCTTCCTTCCGT	2	0.16420361247947454	No Hit
CCCAGCTAGAAAGCTAGCACAGTGAGCAGGGACCTGCCTTGCTCACTGTC	2	0.16420361247947454	No Hit
CTCATCAATAGGAACTGGGCAACCAGGTGGGGAGCCGCCAGGGGAGCTTG	2	0.16420361247947454	No Hit
CGGGGTTCCTGATGCAGATGGACAGATTCATGATCTTCCAGGCCTCCCGC	2	0.16420361247947454	No Hit
CGACAACCTCCTGACAACCTTTAGTGAACAGCTGCTCCCTGAGAAAGGGC	2	0.16420361247947454	No Hit
CACAGCTCCCCCGTCACTCCACGTTCTTGTACTTGGTGAACAGGTTGTAA	2	0.16420361247947454	No Hit
CGGCCTTCTCCGCCCGCAGGGTGGAAGCCTCCCGCCTCAGCGCCGAGTTG	2	0.16420361247947454	No Hit
CTCCAGCAACGCAGCCACTTCATGAAACTACAGGTGGTCAACTCTACAGG	2	0.16420361247947454	No Hit
CAGCAGGGCAATTGGGCTAACAGAGTTGATGGTGAGTGGGTGGCAGTAGT	2	0.16420361247947454	No Hit
CTCCTCATTGCTCATCACATACTTACCTGGGGACAATGAAATAGGTCTTT	2	0.16420361247947454	No Hit
TGCACATTTCCGGGTCTGTGAACCATACACAGACCACAAAGGCCGCTACC	2	0.16420361247947454	No Hit
CTATTTCGGACTGTGTCACCTCAGCCACATTCAGCTTGCACCCTGCCAGT	2	0.16420361247947454	No Hit
GTCCATCTAGTCAATGGGAGAGTACACAGCCACTCCAAAATAAAATATTT	2	0.16420361247947454	No Hit
CTTGCTTTCACTCAGTTCCTATGGACCAGGGTTCCTCTTCAAAGAACCAT	2	0.16420361247947454	No Hit
CTTTCTCCAATCGAGACCCCAAGCAGAGGTTCTCCCACTTTGCCAAGCCA	2	0.16420361247947454	No Hit
CGCGCTTCCCTCACCTATTCAGATACTTGCCGAGTGCCTGCCACTTGCCT	2	0.16420361247947454	No Hit
GTCTGTTCCACGTGGAAGGCCCTACACCCCAATCTTCTCCCCTACTCTGG	2	0.16420361247947454	No Hit
GTCCCAGGTCTCTGCCAGTGAGTCCAGCAGCTTCTGTCCCTCATAGAAGC	2	0.16420361247947454	No Hit
GTGGATGGCAGCCCACAGTTCTCAGAGACGCTGAGTGTGTGGAAGCTGGA	2	0.16420361247947454	No Hit
CTTCTCCGCCCGCAGGGTGGAAGCCTCCCGCCTCAGCGCCGAGTTGGTGA	2	0.16420361247947454	No Hit
CCGAAGCTTCCCTTGCCCCTGCTCCCTCCAGTAGTGGCCCCTTGGGGCTA	2	0.16420361247947454	No Hit
GTACTATTATCTCCATTTTATAGACAAGTCAACTGAGACTCACAGAGGTG	2	0.16420361247947454	No Hit
TGAACATCTTTCATGTGGAGCGATTGTATTTCCTCCTTTGTGGATTGTCA	2	0.16420361247947454	No Hit
AGACAGAAGAGAACTGAGCACAAAGAGAAAGTGATCTGTACACCTCCCAT	2	0.16420361247947454	No Hit
CTGGGACACTCCTGCCACCATCCACAGCTCCCCCGTCACTCCACGTTCTT	2	0.16420361247947454	No Hit
GGGCCTTCCACGTGGAACAGACAGCCCCTGTGTCTCTGTCTCTTGGGGAC	2	0.16420361247947454	No Hit
GGCCAGCCCTCTGCCCACCCGGGTAGCCCAAACCTGGCTCTGAGGGTGAT	2	0.16420361247947454	No Hit
CCGGGATGCCAGCAAGAGGGAGGCGAGCATGCAGTGAGCGATAGGTACAA	2	0.16420361247947454	No Hit
CGAGCACGGAATCTTTAAGAATCCGCCAGCACTTGGTAGCGTCTGCAGCC	2	0.16420361247947454	No Hit
GTCCTTCTTCACGGAGTACCTGCAGAACCAGCTGCTGACAAAAGGCATGG	2	0.16420361247947454	No Hit
GGCAGGAGTGCCATGCATCTCCGATAAGCCAGCTCTGTGGCCTTGGGCTG	2	0.16420361247947454	No Hit
ACCTGCCCTGTGGTCAGCCTGAGATGCAGCCCTTAGTATTACTATTGTTA	2	0.16420361247947454	No Hit
AGCTGAAAGCCCAGCCTTGGCAGCGTCTCCGAATGCCCAGATGGATGACG	2	0.16420361247947454	No Hit
AGCTGGGACACTCCTGCCACCATCCACAGCTCCCCCGTCACTCCACGTTC	2	0.16420361247947454	No Hit
TGAATGGCTGCTGGGACATGAGGCTTGGGTGTGAGATGCCCCGTCTGCTG	2	0.16420361247947454	No Hit
GTCCCAAGCCCACACACATAAGCACCAAGAGAAAAAACACAAATCACCAT	2	0.16420361247947454	No Hit
GGTTAATATCTTTAATATATACCGAGCTCATACAAATTTATCTGACCCCA	2	0.16420361247947454	No Hit
CCTGAATACAGGCTTTAGGTTCCCCTGGACTCCAGCCAAGGCTGTTCAGG	2	0.16420361247947454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
